CANTO II Becton Dickinson


Partner lab price : 21.78 €/hour
Public and private lab price: 59.44 €/hour
Brief description:
Flow cytometry cell analyzer - Becton Dickinson
3 lasers, 2 physical parameters and 8 fluorescences

Controlled by the FACSDiVa software (Becton Dickinson)
Blue laser excitation- 488nm :
Filter 1 (530/30BP) : FITC, AF488, GFP, CFSE, BB515
Filter 2 (556LP - 585/42BP) : PE, PI, Pyronin
Filter 3 (610LP)
Filter 4 (655LP - 670LP) : PerCP, PerCP-Cy5.5, PE-Cy5, PE-Cy5.5, 7-AAD
Filter 5 (735LP - 780/60BP) : PE-Cy7
Acquisition of 10 000 events/second

Intracellular or cell-surface staining, fixed or alive cells

L2 laboratory completely equiped : hood, centrifuge, pipets
Red laser excitation - 633nm :
Filter 6 (660/20BP) : APC, AF647, Cy5, TOPRO-3
Filter 7 (685LP)
Filter 8 (735LP - 780/60BP) : APC-Cy7, APC-H7, APC-AF750, APC-eF780

Violet laser excitation - 405nm :
Filter 9 (450/50) : BD Horizon V450, Pacific Blue, AF405, DAPI, BV421
Filter 10 (502LP - 510/50BP) : BD Horizon V500, Pacific Orange, AmCyan, BV510
Ideal operating temperatures between 19 and 26°C