Zeiss LSM780 confocal microscope

Tariff (public) : 37.04 €/hour
Tariff (private) : -- €/hour

Brief description:
State-of-the-art fully motorized, Zen software-driven, inverted confocal microscope for epifluorescence and transmission imaging.
Fluorescence (405, Argon, DPSS 561 and HeNe 633 LASERs) and brightfield/DIC.
Multidimensional acquisitions (X,Y,Z, spectral and time), F-techniques, incubation.
10X Plan NEOFLUAR 0.3 NA
20X Plan Apochromat 0.8 NA
40X Plan Apochromat 1.3 NA oil DIC
63X Plan Apochromat 1.4 NA oil DIC

Detectors :
2 multialkaline photocathodes PMTs (including 1 cooled PMT)
1 transmission PMT
1 32 multichannel cooled PMT with a GaAsP photocathode for spectral detection

BSL1 environment
Illumination light source for widefield sample localization:
Fibered metal halide arc source for episcopic illumination
100W tungsten bulb for diascopic illumination

Binocular tube, X, Y and Z stage, fluorescence filter set turret, transmission mirror, objective turret

Fluorescence filter sets for widefield sample localization:
Available acquisition techniques :
405, 458, 476, 488, 514, 561 et 633nm LASER excitation
2 and 3D mosaic acquisition (full slide scan, local scans per position) , field rotation, spectral (offline or online separation). Hardware or software automatic focusing.
Any photoactivation/destruction experiment (FRAP, PA, FLIP), ratiometric FRET analysis.

User-defined protocols
Image quality:
Confocal technology allows improved spatial resolution and contrast compared to widefield imaging. Spatial resolution may be further improved using deconvolution algorithms (Huygens).

Sensitivity :
The central, multichannel PMT design allows a x2 to x3 improvement in photon collection efficiency compared to regular photocathode PMT
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