Abberior STED superresolution microscope


Tariff (public) : -- €/hour
Tariff (private) : -- €/hour
Associated technologies:
  • 2D imaging
  • 3D imaging
  • High resolution

  • Brief description:
    Inverted microscope, fully motorized, controlled by Imspector software (Abberior). This microscope allows super resolution imaging in 2D STED or 3D STED, up to 3 colors.
    Excitation LASERS 405, 485, 561 and 640nm.
    STED LASERS 595nm & 775nm.
    Objectives :
    100X Plan SuperApochromat 1.4 Oil
    100X Plan SuperApochromat 1.35 Sil
    60X Plan Apochromat 1.2 W
    20X Plan Apochromat 0.75

    Detectors :
    4 Avalanche Photo Diodes & spectral detection.

    Autofocus & Z-drift compensation

    RESCue imaging mode in confocal & STED

    L1 environement

    For more information, contact us : This email address is being protected from spambots. You need JavaScript enabled to view it.
    2D & 3D STED

    Incubation :
    Stage Top Incubator : Fully controlled environment including temperature, pH (CO2) & hygrometry. Objective heater.

    Deltavision OMX

    
    Tariff (public) : 37.04 €/hour
    Tariff (private) : -- €/hour

    Brief description:
    Inverted, fully-motorized, softWoRx software-controlled microscope for fluorescence and transmission imaging. This workstations allows either high speed (+TIRF, photomanipulation) or 3D-SIM & 2DPALM/STORM superresolution imaging.
    405, 488, 561 & 642nm LASERs and LED for Brightfield/DIC imaging
    Objectives:
    60x Plan Apochromat 1.3 Sil
    100x Plan SuperApochromat 1.4 Oil (DIC)

    Detectors:
    4 hight quantum efficiency photometrics Evolve 512B EMCCD cameras (512x512, effective pixels size of 132nm with 60x lens & 79nm with 100x lens).

    Incubation:
    Fully controlled environment including temperature, pH (CO2), oxygen concentration & hygrometry. Objective heater for 60x lens.

    BSL1 environment
    Available imaging techniques:
    The device may be used in two distinct mode
    - Highspeed imaging mode: 2 or 3D multiposition along time (up to a simultaneous 4 colors stack of 15 planes/sec). Hardware autofocus. Ratiometric FRET imaging, TIRF, Photomanipulation (FRAP/PA).

    - Superresolution mode for a spatial resolution of 120x120x300nm (3D-SIM mode) and 2D PALM/STORM multiposition mode available.
    Light sources:
    405, 488, 561 (3 colors 3D-SIM) & 642nm LASERs excitation (4 colors highspeed imaging) and white LED (diascopic illumination)

    Dichroic for fluorescence imaging:
    East camera: 419-465nm
    West camera: 500-550nm
    North East camera: 581-618nm
    North West camera: 664-704nm

    Neutral density filters for LASER attenuation:
    0.1%, 1%, 10%, 30%, 100%
    Sample compatibility :
    25mm coverslips (to be mounted in a chamber), 35mm glass-bottom dishes, thin-bottom Labtek chambers

    Image quality:
    For 3D-SIM imaging, sample preparation best practice should be followed. The coverslip thickness and mounting medium RI should be controlled. Spherical aberrations can be attenuated using different RI immersion oils.
    Inquiries may be sent to This email address is being protected from spambots. You need JavaScript enabled to view it.