MuViSPIM


Tarif établissement partenaire: 15.66 €/heure
Tarif établissement public et privé : 55.41 €/heure
Technologies associées :
  • Imagerie 3D
  • Imagerie in vivo

    • Brève description :
    Luxendo Light Sheet microscope for 3D live imaging of small organisms (such as embryos). The set-up has 2 illumination arms with 4 laser lines (488, 515, 561 and 642nm) and 2 emission arms with 2 emission objectives (33.3X final magnification).

    Controlled by Luxendo software
    Objectives:
    10X Plan FLUOR 0.3NA Water Nikon *2
    20X XLUMPlanFL N 1.0NA Water Olympus *2

    Detectors:
    2 sCMOS Hamamatsu Orca Flash 4 V2 cameras
    (2048*2048, 6.5µm pixel size)

    Incubation:
    temperature between 10 and 40°C and CO2 control
    Acquisition modes:
    Light sheet imaging in multi-channels mode (widefield or confocal), in multi-Z stacks, in multi-timepoints, in multi-angles with the sample rotation
    Z-piezo stage with 20nm resolution, XY stage with 100nm resolution and sample rotation in 360° angle
    Illumination sources:
    laser 488nm
    laser 515nm
    laser 561nm
    laser 642nm
    Light sheet generated with a digital light scanner

    Spectral selection:
    5 emission filters in a filter wheel
    GFP (488 LP)
    GFP en combinaison (525/50 BP)
    YFP (535/30 BP)
    Cherry (561 LP)
    FarRed (642 LP)
    Sample carriers:
    the sample is prepared in a glass capillary either embedded in a gel or loaded in a well formed in the gel. This capillary is charged in the sample carrier on the stage.
    Each project done on this MuViSPIM needs specific development for the sample preparation