summary and conclusions

• image from microscope is degraded

• it contains noise and blur

• blur can be described as a convolution of object function and psf

• image nearer to the object function can be obtained by image restoration yielding higher resolution and better contrast

• MLE is a deconvolution algorithm approriate for fluorescent microscope images

• imaging process is not finished finished without deconvolution

• do it whenever high quality images are needed