Microscope parameter

• sample medium

• refractive index n

  • default (all media for example water) 1,33810
  • liquid Vectashield (not polymerized) 1,49000
  • 90-10 (v:v) glycerol - PBS ph 7.4 1,49000
  • prolong antifade 1,4

• limits the NA and therefore the possible resolution