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Partner lab price : 48.99 €/h
Public and private lab price131.03 €/h
 
Brief description :
Flow cytometry cell sorter: spectral technology and associated real-time imaging (CellView). Becton Dickinson.
Controlled by FacsChorus software.

 

Fluorescence: Spectral technology with 56 APD (avalanche photodiode) detection channels. 4 LASERs for fluorochrome excitation: violet (405nm, 50mW), blue (488nm, 100mW), yellow (561 nm, 50mW), red (640nm, 100mW).

Imaging: possible on 3 fluorescence channels linked to blue laser excitation. Analysis of parameters based on cell morphology, interaction between 2 cells, localization of one or more markers within a cell. Can be used for sorting or sample quality control.

3 different nozzle sizes :
-85-μm nozzle: 35 psi, 57 KHz
-100-μm nozzle: 20 psi, 34 KHz
-130-μm nozzle: 7 psi, 15 KHz

High-speed cell sorting on 2, 4 or 6 ways, cloning on 6, 12, 24, 96 or 384-well plates and slides.

Possibility of cooling or heating samples and sorted fractions.

Sterile sorting with possibility of culturing recovered cells. Sorting under PSM class II.

Intracellular or surface labeling, fixed or live.

Ideal operating temperatures between 20 and 24°C.

Laboratory in L2 containment equipped with PSM, refrigerator, centrifuge, small equipment.