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Partner lab price: 15.66 €/h
Public and private lab price: 55.41 €/h
Technologies:
- 2D imaging
- 3D imaging
Short description:
Upright widefield epifluorescence microscope ideal for 2D or 3D acquisitions followed by deconvolution, for fixed samples, calcium imaging, eye imaging in vivo.

Image quality improvement with software technology THUNDER.
Multi-dimensional acquisition
Acquisition software: LASX

 

Objectives:
1.6x/0.05 HC PL Fluotar
10X/0.32 HC PL Fluotar
16X/0.60 HC Fluotar L IMM CORR
20X/0.55 HC PL Fluotar
20X/0.80 HC PL APO
40X/0.95 HC PL APO CORR
63X/1.40 Oil HC PL APO

20X/0.80 Oil HC PL Fluotar (on demand for Fura)

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Light sources:
- Fluorescence: pe-800 fura (wavelengths (nm): 340, 380, 435, 470, 500, 550, 580, 635)
- Transmitted light: white LED

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Filter cubes: 3 multiband cubes
- QL1: DAPI, GFP, AF594, AF647
- CYR: CFP, YFP, RFP
- Fura: 340/380 (calcium probe)

Cameras:
- for fluorescence: B&W K8 sCMOS 4.2MP (2048x2048, pixel size = 6.5µm)
- for transmitted light: K3C color CMOS 6.3 MP (3072x2048, pixel size = 2.4 µm)

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Acquisition types:
- Automated on samples, from 1 to 4 slides: XYZT, multichannel, mosaics
- Navigator module and external filter wheel to improve acquisition speed
- Calcium imaging
Customizable protocol

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Improvement of image quality with THUNDER software technology, during or after acquisition