Inverted Nikon

Partner lab price : 15.66 €/hour
Public and private lab price: 55.41 €/hour
Associated technologies:
  • 2D imaging
  • Videomicroscopy

  • Brief description:
    Inverted Nikon TiE wide-field microscope for timelapse in 2D or 3D in fluorescence or transmitted light mode (BF, DIC). Trigger option for fast imaging.

    Controled by NIS Elements.
    10X Plan Apochromat 0.45NA air
    20X Plan Apochromat 0.75NA air
    40X Plan Fluor 1.3NA oil
    60X Plan Apochromat 1.4NA oil
    100X Plan Apochromat 1.40NA oil

    1 camera sCMOS back-illuminated Prime95B Photometrics
    (1200*1200, 11µm pixel size)
    temperature and CO2 control

    Type of acquisition :
    -widefield epifluorescent microscopy
    -transmitted light microscope in bright field or DIC mode.

    PFS module for control of the z drift during timelapse.

    Trigger option for fast multicolor imaging.
    Illumination source:
    Lumencor with 7 LED and excitation filters for:
    DAPI, CFP, GFP, YFP, Cy3, Cherry, Cy5

    Emission filters:
    DAPI, CFP, GFP, YFP, Cy3, Cherry, Cy5
    Compatible sample holders:
    35mm dish with #1.5 glass bottom, glass slide with #1.5 glass coverslip or glass-bottom multi-well plate