Inverted Nikon


Partner lab price : 15.66 €/hour
Public and private lab price: 55.41 €/hour
Associated technologies:
  • 2D imaging
  • Videomicroscopy

  • Brief description:
    Inverted Nikon TiE wide-field microscope for timelapse in 2D or 3D in fluorescence or transmitted light mode (BF, DIC). Trigger option for fast imaging.

    Controled by NIS Elements.
    Objectives:
    10X Plan Apochromat 0.45NA air
    20X Plan Apochromat 0.75NA air
    40X Plan Fluor 1.3NA oil
    60X Plan Apochromat 1.4NA oil
    100X Plan Apochromat 1.40NA oil

    Detectors:
    1 camera sCMOS back-illuminated Prime95B Photometrics
    (1200*1200, 11µm pixel size)
    Incubation:
    temperature and CO2 control

    Type of acquisition :
    -widefield epifluorescent microscopy
    -transmitted light microscope in bright field or DIC mode.

    PFS module for control of the z drift during timelapse.

    Trigger option for fast multicolor imaging.
    Illumination source:
    Lumencor with 7 LED and excitation filters for:
    DAPI, CFP, GFP, YFP, Cy3, Cherry, Cy5

    Emission filters:
    DAPI, CFP, GFP, YFP, Cy3, Cherry, Cy5
    Compatible sample holders:
    35mm dish with #1.5 glass bottom, glass slide with #1.5 glass coverslip or glass-bottom multi-well plate