Spinning disk Dragonfly


Tariff (public) : 17.47 €/hour
Tariff (private) : -- €/hour
Associated technologies:
  • 3D imaging
  • Videomicroscopy

  • Brief description:
    Nikon inverted micorsopce coupled to the Andor Dragonfly spinning disk dedicated to 3D confocal imaging of live samples or fixed samples. This microscope is also equiped 2 cameras, for simultaneous 2 color live imaging.

    Controlled by the Andor fusion software.
    Objectives:
    10X Plan Apo lambda 0.45 NA 4mm WD dry
    20X Plan Apo lambda 0.75 NA 1mm WD oil
    40X Plan Fluor 1.3 NA 0.2mm WD oil
    40X Apo 1.15 NA 0.6mm WD water
    60X Plan Apo lambda 1.4 NA 0.13mm WD oil
    100X Plan Apo lambda 1.45 NA 0.13mm WD oil

    Detector:
    2 camaras EMCCD iXon888 Life Andor
    (1024*1024 pixels, 13µm pixel size, 2x lens if needed, QEmax 95%)

    Environmental control: temperature and CO2
    Illumination sources:
    1 laser bench for confocal mode:
    405nm (100mW), 488nm (150mW), 561nm (100mW) et 637nm (140mW)

    Spectral configuration:
    2 dichroïcs in the head unit: either 100% to one camera or DAPI/GFP for vertical camera and mCherry/Cy5 for other camera with 565 dichroic mirror
    6 emission filters in the external wheel:
    DAPI (450/50), GFP (525/50), mCherry (620/60), Cy5 (700/775), GFP/mCher
    Type of acquisition:
    multi-dimensional acquisition in bright field or DIC transmission and confocal in fluorescence: XYZ (Z piezo of range 300um), multi-channels, multi-position, tile-scans, multi-wells, timelapse
    hardware autofocus (PFS)

    Compatible sample holders:
    35mm dish with #1.5 glass bottom, glass slide with #1.5 glass coverslip or glass-bottom multi-well plate