Opera Phenix

Partner lab price : 37.04 €/hour
Public and private lab price: 101.89 €/hour
Associated technologies:
  • High Content Screening
  • Image analysis

  • Brief description:
    Automated epifluorescent widefield and confocal (spinning disk) microscope with 2 cameras. Ideal for high throughput measure and analysis of cellular content with a large number of samples (cellules, sphéroïdes...) fixed or live and in microplate format.
    Piloted by Harmony software
    5X air 0.16NA Plan Apochromat
    10X air 0.3NA EC Plan Neofluar
    20X air O.4NA LD Plan Neofluar
    20X air O.8NA Plan Apochromat
    63X eau 1.15NA LD C-Apochromat

    Twister III robotic arm to automatically charge the microplates
    Excitation Source:
    4 Lasers : 405 nm, 488 nm, 561 nm et 640 nm

    Emission filters:
    435-550 nm, -435-480 nm, 500-550 nm, 570-630 nm et 650-760 nm

    Can read any format of microplate (6 à 1536 wells)
    2 cameras sCMOS 4.7Mpx 16bits
    (2160 x 2160 pixels, 6.5µm pixel size)

    Environmental control: temperature and CO²
    -multidimensional automated acquisition: YXZ, multi-channels, multi-fileds, multi-wells, multi-plates, timelaps
    -on-the-fly image analysis: personnalized protocol based on different building blocks pipeline
    -Preciscan XYZ: detection of rare objects or events at low magnification and automated ReScan at higher magnification
    -Analysis and classification of objects by Machine Learning