Opera Phenix


Partner lab price : 37.04 €/hour
Public and private lab price: 101.89 €/hour
Associated technologies:
  • High Content Screening
  • Image analysis

    • Brief description:
    Automated epifluorescent widefield and confocal (spinning disk) microscope with 2 cameras. Ideal for high throughput measure and analysis of cellular content with a large number of samples (cellules, sphéroïdes...) fixed or live and in microplate format.
    Piloted by Harmony software
    Objectives:
    5X air 0.16NA Plan Apochromat
    10X air 0.3NA EC Plan Neofluar
    20X air O.4NA LD Plan Neofluar
    20X air O.8NA Plan Apochromat
    63X eau 1.15NA LD C-Apochromat

    Twister III robotic arm to automatically charge the microplates
    Excitation Source:
    4 Lasers : 405 nm, 488 nm, 561 nm et 640 nm

    Emission filters:
    435-550 nm, -435-480 nm, 500-550 nm, 570-630 nm et 650-760 nm

    Can read any format of microplate (6 à 1536 wells)
    Detector:
    2 cameras sCMOS 4.7Mpx 16bits
    (2160 x 2160 pixels, 6.5µm pixel size)

    Environmental control: temperature and CO²
    Applications:
    -multidimensional automated acquisition: YXZ, multi-channels, multi-fileds, multi-wells, multi-plates, timelaps
    -on-the-fly image analysis: personnalized protocol based on different building blocks pipeline
    -Preciscan XYZ: detection of rare objects or events at low magnification and automated ReScan at higher magnification
    -Analysis and classification of objects by Machine Learning