Public and private lab price : €/h
2D imaging
3D imaging
FRAP Photoactivation Photoconversion
Laser ablation
Bruker upright microscope coupled with two Coherent lasers, enabling cellular and tissue imaging of live or fixed samples, and integrated with electrophysiology. Prairie View acquisition software.
Objective: 20X Olympus XLUMPLFLN (N.A. 1) water immersion, WD 2 mm.
Detectors: 3 GaAsP NDD detectors.
Acquisition modes: XYZ multi-photon confocal imaging, time-course, multi-position or mosaic. Photostimulation/photoactivation experiments. The system is equipped with a galvanometric scanner and a resonant scanner. Multi-photon imaging can be coupled with electrophysiological recordings.
Customisable protocols.
Excitation sources: Chameleon Discovery NX (Coherent) dual-line laser: one tunable line in the 660–1320 nm range and one fixed line at 1040 nm. A delay line enables the temporal synchronisation of the tunable and fixed laser beams.
Spectral configuration:
For two-photon imaging, an LP 565 dichroic filter combined with BP 525/70 and BP 595/50 filters, and an LP 495 dichroic filter combined with a BP 485/25 filter.
Full-field observation: fluorescence and transmission. A GFP cube (470/40, 495LPXR, 525/50) and a DsRed cube (545/30, 570LP, 620/60) combined with a Retiga Electro CCD camera for observation and navigation across the sample.
Sample holders: Slice Mini bathchamber II (Luigs&Neumann).
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