Public and private lab price : 82.24 €/h
2D imaging
3D imaging
FRAP Photoactivation Photoconversion
Videomicroscopy
Nikon inverted microscope coupled with a Yokogawa CSU-W1 spinning-disc head, designed for 3D confocal imaging of live samples. The system is controlled by NIS software.
Motorised stage.
Objectives
4x PlanApo Lambda D (N.A. 0.2) air
25x Plan Apo Lambda S (N.A. 1.05) silicone immersion
40x Plan Apo Lambda D (N.A. 0.95) air immersion
60x Super Plan Fluor ELWD (N.A. 0.70) air immersion
60x Plan Apo Lambda D (N.A. 1.42) oil immersion
100x Plan Apo Lambda D (N.A. 1.45) oil immersion
100x Plan Apo Lambda S (N.A. 1.35) silicone immersion
Detectors:
Hamamatsu Orca Fusion BT sCMOS camera (2304 x 2304, pixel size 6.5 µm)
Andor iXon Life 888 EMCCD camera (1024 x 1024, pixel size 13 µm)
Environmental control: temperature and CO₂
Acquisition types: multi-dimensional transmitted light, DIC and confocal fluorescence acquisitions: XYZ, multi-channel, multi-position, mosaic, multi-well, time-lapse. TIRF and FRAP (ILAS 2).
Focus tracking (PFS)
Customisable protocols
Excitation sources: 405 nm (120 mW), 445 nm (100 mW), 488 nm (200 mW), 515 nm (100 mW), 561 nm (150 mW) and 638 nm (200 mW) lasers.
Spectral configuration:
Dichroic filters in the head: triple-band 445/515/561, quad-band 405/488/561/640
Emission filters for the CMOS camera: DAPI (447/60), GFP (525/30), YFP (524/27), Cy3 (600/52), Cy5 (708/75), Dual GFP/dsRed (532/610)
Emission filters for the EMCCD camera: DAPI (447/60), CFP (472/30), GFP (525/30), YFP (524/27), Cy3 (600/52), Cy5 (708/75), Dual GFP/dsRed (532/610)
Compatible sample holders: 35mm boxes with #1.5 glass cover slip base, #1.5 glass cover slip mounted on a slide, multi-well plate with #1.5 glass cover slip base.
For further information, please contact