Zeiss Axioimager Z3 Apotome
Fluorescence and brightfield/darkfield/polarisation (w/b and color large field size cameras).
For any multidimensional acquisition (X,Y,Z, lambda and t).
Partner lab price : 15.66 €/hour
Public and private lab price: 55.41 €/hour
Brief description:
State-of-the-art, fully motorized, zen software-driven, upright widefield epifluorescence microscope equipped with an apotome slider. Fluorescence and brightfield/darkfield/polarisation (w/b and color large field size cameras).
For any multidimensional acquisition (X,Y,Z, lambda and t).
Objectives:
5X Plan Apochromat 0.16
10X Plan Apochromat 0.45 NA
20X Plan Apochromat 0.8 NA
40X Plan Apochromat 1.4 NA oil
63X Plan Apochromat 1.46 NA oil Corr
Detectors:
1 ORCA-Flash4 LT camera Hamamatsu monochrome
(2048 x 2048 pixels, 6.5µm pixel size)
1 ZEISS quadriCCD Axiocam 506 6MP color camera
(2752 × 2208 pixels, 4.54µm pixel size)
BSL1 environment
5X Plan Apochromat 0.16
10X Plan Apochromat 0.45 NA
20X Plan Apochromat 0.8 NA
40X Plan Apochromat 1.4 NA oil
63X Plan Apochromat 1.46 NA oil Corr
Detectors:
1 ORCA-Flash4 LT camera Hamamatsu monochrome
(2048 x 2048 pixels, 6.5µm pixel size)
1 ZEISS quadriCCD Axiocam 506 6MP color camera
(2752 × 2208 pixels, 4.54µm pixel size)
BSL1 environment
Light sources:
XCite 120LED, Zen-driven LED light source for episcopic illumination.
White Carl Zeiss LED source for diascopic illumination
Motorization:
Trinocular tube, stage (X, Y and Z), fluorescence filter set turret, condenser turret, objective turret, apotome slider.
Fluorescence filter sets:
Hoechst
GFP
Cy3
Texas Red
Cy5
Polariser +/- a green filter for Picrosirius Red imaging.
XCite 120LED, Zen-driven LED light source for episcopic illumination.
White Carl Zeiss LED source for diascopic illumination
Motorization:
Trinocular tube, stage (X, Y and Z), fluorescence filter set turret, condenser turret, objective turret, apotome slider.
Fluorescence filter sets:
Hoechst
GFP
Cy3
Texas Red
Cy5
Polariser +/- a green filter for Picrosirius Red imaging.
Available acquisition techniques:
Any slide acquisition automation in both fluorescence and transmission modes. Images acquired with both color and w/b cameras are automatically realigned.
Mosaic acquisition (full slide scan, slices on a slide scan) with user defined autofocus mode and autofocus map.
User-defined protocols
Any slide acquisition automation in both fluorescence and transmission modes. Images acquired with both color and w/b cameras are automatically realigned.
Mosaic acquisition (full slide scan, slices on a slide scan) with user defined autofocus mode and autofocus map.
User-defined protocols
Image quality:
Both axial resolution and contrast in epifluorescence mode may be improved using the Grid Projection Illumination technique (aka "apotome"). The grid lines spacing is automatically set (motorized apotome slide). The spatial resolution may be further improved using deconvolution (Huygens or Zen software).
Both axial resolution and contrast in epifluorescence mode may be improved using the Grid Projection Illumination technique (aka "apotome"). The grid lines spacing is automatically set (motorized apotome slide). The spatial resolution may be further improved using deconvolution (Huygens or Zen software).
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