Zeiss Axioimager Z3 Apotome


Tariff (public) : 15.66 €/hour
Tariff (private) : -- €/hour
Associated technologies:
  • 2D imaging
  • 3D imaging

  • Brief description:
    State-of-the-art, fully motorized, zen software-driven, upright widefield epifluorescence microscope equipped with an apotome slider.
    Fluorescence and brightfield/darkfield/polarisation (w/b and color large field size cameras).
    For any multidimensional acquisition (X,Y,Z, lambda and t).
    Objectives:
    5X Plan Apochromat 0.16
    10X Plan Apochromat 0.45 NA
    20X Plan Apochromat 0.8 NA
    40X Plan Apochromat 1.4 NA oil
    63X Plan Apochromat 1.46 NA oil Corr

    Detectors:
    1 ANDOR sCMOS ZYLA 4.2 MP monochrome camera
    (2048 x 2048 pixels, 6.5µm pixel size)
    1 ZEISS quadriCCD Axiocam 506 6MP color camera
    (2752 × 2208 pixels, 4.54µm pixel size)

    BSL1 environment
    Light sources:
    XCite 120LED, Zen-driven LED light source for episcopic illumination.
    White Carl Zeiss LED source for diascopic illumination

    Motorization:
    Trinocular tube, stage (X, Y and Z), fluorescence filter set turret, condenser turret, objective turret, apotome slider.

    Fluorescence filter sets:
    Hoechst
    GFP
    Cy3
    Texas Red
    Cy5
    Polariser +/- a green filter for Picrosirius Red imaging.
    Available acquisition techniques:
    Any slide acquisition automation in both fluorescence and transmission modes. Images acquired with both color and w/b cameras are automatically realigned.
    Mosaic acquisition (full slide scan, slices on a slide scan) with user defined autofocus mode and autofocus map.

    User-defined protocols
    Image quality:
    Both axial resolution and contrast in epifluorescence mode may be improved using the Grid Projection Illumination technique (aka "apotome"). The grid lines spacing is automatically set (motorized apotome slide). The spatial resolution may be further improved using deconvolution (Huygens or Zen software).
    Inquiries may be sent to This email address is being protected from spambots. You need JavaScript enabled to view it.