Partner lab price : 15.66 €/hour
Public and private lab price: 55.41 €/hour

2D imaging

3D imaging

Upright widefield microscope for 2D or 3D acquisition dedicated to fixed samples between slide and coverslip
B&W Caméra CMOS Orca Flash 4.0
XYZ motorized stage
Fluorescence, Brightfield,
Apotome 2 module

Software : Zen by Zeiss

Objectives:
10X Plan Apochromat / 0.45 NA
20X Plan Apochromat / 0.8 NA
40X Plan Apochromat 1.3 NA. oil
63X Plan Apochromat 1.4 NA. oil
100X Plan Neofluar 1.3 NA. oil

Illumination source for fluorescence : HXP-120

Fluorescence filter cubes:
Dapi. Ex : 335-383. Em :420-470
Alexa Fluor 489 Ex : 450-490. Em :515-565
DsRed Ex : 533-558. Em :570-640
HE mPlum Ex : 574-599. Em :612-682
Cy5 Ex : 625-655. Em :665-715

Detector:
B&W Caméra CMOS Orca Flash 4.0 (Hamamatsu) for large field and high resolution (2048 x 2048; pixel size 6.5µm)
Apotome 2 module

Prestations MRI sur les systemes d'imagerie
Surveillance et maintenance

Partner lab price : 15.66 €/hour
Public and private lab price: 55.41 €/hour

2D imaging

3D imaging

Videomicroscopy

State-of-the-art, fully motorized, zen software-driven, upright widefield epifluorescence microscope equipped with an apotome slider and DIC optics.
Fluorescence and brightfield/darkfield/polarisation/DIC (w/b large field size camera).
For any multidimensional acquisition (X,Y,Z, lambda and t).

Objectives:
5X Plan Apochromat 0.16
10X Plan Apochromat 0.45 NA DIC
20X Plan Apochromat 0.8 NA DIC
40X Plan Apochromat 1.3 NA oil DIC
63X Plan Apochromat 1.4NA oil
100X Plan Apochromat 1.46 oil DIC

Detector:
1 ORCA-Flash4 LT Hamamatsu monochrome camera
(2048 x 2048 pixels, 6.5µm pixel size)

BSL1 environment

Light sources:
XCite 120LED, Zen-driven LED light source for episcopic illumination.
White Carl Zeiss LED source for diascopic illumination

Motorization:
Binocular tube, stage (X, Y and Z), fluorescence filter set turret, condenser turret, objective turret, apotome slider.

Fluorescence filter sets:
Hoechst
GFP HE
Cy3
Texas Red
Cy5
Polariser +/- a green filter for Picrosirius Red imaging.

Available acquisition techniques:
Any slide acquisition automation in both fluorescence and transmission modes. Images acquired with both color and w/b cameras are automatically realigned.
Mosaic acquisition (full slide scan, slices on a slide scan) with user defined autofocus mode and autofocus map.

User-defined protocols

Image quality:
Both axial resolution and contrast in epifluorescence mode may be improved using the Grid Projection Illumination technique (aka "apotome"). The grid lines spacing is automatically set (motorized apotome slide). The spatial resolution may be further improved using deconvolution (Huygens or Zen software).

Inquiries may be sent to This email address is being protected from spambots. You need JavaScript enabled to view it.

Prestations MRI sur les systemes d'imagerie
Surveillance et maintenance

Partner lab price : 15.66 €/hour
Public and private lab price: 55.41 €/hour

2D imaging

3D imaging

State-of-the-art, fully motorized, zen software-driven, upright widefield epifluorescence microscope equipped with an apotome slider.
Fluorescence and brightfield/darkfield/polarisation (w/b and color large field size cameras).
For any multidimensional acquisition (X,Y,Z, lambda and t).

Objectives:
5X Plan Apochromat 0.16
10X Plan Apochromat 0.45 NA
20X Plan Apochromat 0.8 NA
40X Plan Apochromat 1.4 NA oil
63X Plan Apochromat 1.46 NA oil Corr

Detectors:
1 ORCA-Flash4 LT camera Hamamatsu monochrome
(2048 x 2048 pixels, 6.5µm pixel size)
1 ZEISS quadriCCD Axiocam 506 6MP color camera
(2752 × 2208 pixels, 4.54µm pixel size)

BSL1 environment

Light sources:
XCite 120LED, Zen-driven LED light source for episcopic illumination.
White Carl Zeiss LED source for diascopic illumination

Motorization:
Trinocular tube, stage (X, Y and Z), fluorescence filter set turret, condenser turret, objective turret, apotome slider.

Fluorescence filter sets:
Hoechst
GFP
Cy3
Texas Red
Cy5
Polariser +/- a green filter for Picrosirius Red imaging.

Available acquisition techniques:
Any slide acquisition automation in both fluorescence and transmission modes. Images acquired with both color and w/b cameras are automatically realigned.
Mosaic acquisition (full slide scan, slices on a slide scan) with user defined autofocus mode and autofocus map.

User-defined protocols

Image quality:
Both axial resolution and contrast in epifluorescence mode may be improved using the Grid Projection Illumination technique (aka "apotome"). The grid lines spacing is automatically set (motorized apotome slide). The spatial resolution may be further improved using deconvolution (Huygens or Zen software).

Inquiries may be sent to This email address is being protected from spambots. You need JavaScript enabled to view it.

Prestations MRI sur les systemes d'imagerie
Surveillance et maintenance

Partner lab price : 5.36 €/hour
Public and private lab price: 22.32 €/hour

Macroscope with a zoom, 2 LED sources for brightfield images and 1 source for fluorescence image. Zoom, focus and stage motorized.
System ideal for screening/phenotyping of large samples, a wide variety of sample supports (dish, slide, multi-well plate...)

Controled by Zen software

Objectives:
1X 0.25 NA PlanNeoFluar Z
2.3 X 0.57 NA PlanNeoFluar Z

Excitation source:
HXP 200 lamp

Filter cubes:
DAPI : ex 365 ; em 420
CFP : ex 436/20 ; em 480/40
GFP : ex 470/40 ; em 525/50
YFP : ex 500/20 ; em 535/30
Cy3 : 545/25 ; em 605/70
Texas Red : 560/40 ; em 630/75

Cy5 filter available on demand

Detectors:
1 colour Axiocam 305 camera
(2464 X 2056 pixels ; pixel size = 3.45µm)
1 monochrome Hamamatsu Orca Flash 4.0LT camera
(2048 X2048 pixels ; pixel size= 6.5 µm)

Acquisition mode:
Brightfield, darkfield, oblique imaging and also fluorescence imaging.
Multichannel / Z stack / Tile & Positions (mosaics) / Auto Focus
Module Z Extended Focus: processing of Z-stack without limitation of field depth (improved projection)

L1 environment

Prestations MRI sur les systemes d'imagerie
Surveillance et maintenance