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Abberior STED superresolution microscope


Partner lab price : -- €/hour
Public and private lab price: -- €/hour
Associated technologies:
  • 2D imaging
  • 3D imaging
  • High resolution

  • Brief description:
    Inverted microscope, fully motorized, controlled by Imspector software (Abberior). This microscope allows super resolution imaging in 2D STED or 3D STED, up to 3 colors.
    Excitation LASERS 405, 485, 561 and 640nm.
    STED LASERS 595nm & 775nm.
    Objectives :
    100X Plan SuperApochromat 1.4 Oil
    100X Plan SuperApochromat 1.35 Sil
    60X Plan Apochromat 1.2 W
    20X Plan Apochromat 0.75

    Detectors :
    4 Avalanche Photo Diodes & spectral detection.

    Autofocus & Z-drift compensation

    RESCue imaging mode in confocal & STED

    L1 environement

    For more information, contact us : This email address is being protected from spambots. You need JavaScript enabled to view it.
    2D & 3D STED

    Incubation :
    Stage Top Incubator : Fully controlled environment including temperature, pH (CO2) & hygrometry. Objective heater.

    About MRI-CIA

    The MRI Center for Image Analysis (MRI-CIA)

    MRI-CIA has been created to answer the bioimage analysis needs of the users of MRI. We offer comprehensive support for bioimage analysis, from the start of a research project to publication. The support we provide is tailored to each individual case and can include one-on-one software training or the creation of custom analysis tools. We handle various aspects of image analysis, such as for example (list non-comprehensive):

    • image data handling
    • image restoration and registration
    • quantitative image analysis and visualization
    • instance segmentation and tracking
    • morphological and intensity quantification
    • colocalization analysis and spatial statistics

    MRI-CIA closely collaborates with the other engineers of the MRI core facility in order to ensure optimal image acquisition modalities for downstream quantitative analysis.

    Contact: This email address is being protected from spambots. You need JavaScript enabled to view it.

    Please find more information here: The MRI image analysis service.pdf

    ARIA IIu Becton Dickinson

    
    Partner lab price : 48.99 €/hour
    Public and private lab price: 131.03 €/hour
    Brief description:
    Analyzer and cell sorter by flow cytometry - Becton Dickinson
    4 lasers, 2 physical parameters and 17 fluorescences

    Controlled by the FACSDiVa software (Becton Dickinson)
    Blue laser excitation- 488nm :
    Filter 1 (505LP - 530/30BP) : FITC, AF488, GFP, CFSE, BB515
    Filter 2 (550LP - 575/25BP) : PE
    Filter 3 (600LP - 610/20BP) : mCherry, PI, PE-TexasRed, PE-CF594
    Filter 4 (635LP - 660/20BP) : PE-Cy5, 7-AAD
    Filter 5 (685LP - 710/50BP) : PerCP, PerCP-Cy5.5, PerCP-eF710
    Filter 6 (755LP - 780/60BP) : PE-Cy7
    Violet laser excitation - 405nm :
    Filter 10 (450/50BP) : BD Horizon V450, Pacific Blue, AF405, BV421
    Filter 11 (505LP - 525/50BP) : BD Horizon V500, Pacific Orange, AmCyan, Qdot525, BV510
    Filter 12 (595LP - 610/20BP) : Qdot605, BV605, eF605NC, eF625NC
    Filter 13 (630LP - 670/30BP) : Qdot655, BV650, eF650NC
    Filter 14 (685LP - 710/50BP) : Qdot700, BV711
    Filter 15 (750LP - 780/60BP) : Qdot800, BV785
    Red laser excitation - 633nm :
    Filter 7 (660/20BP) : APC, AF647
    Filter 8 (690LP - 730/45BP) : AF700, APC-R700
    Filter 9 (755LP - 780/60BP) : APC-Cy7, eFluor780, AAF

    UV laser Excitation - 355nm :
    Filter 16 (450/50BP) : DAPI, Hoechst, Hoechst 33258, Hoechst Blue, Indo-1 (violet)
    Filter 17 (505LP - 530/30BP) : Hoechst Red, Indo-1 (blue)
    High speed cell sorting (up to 25000 events/sec) on 2 or 4 ways, ACDU (automatic cell deposition unit) for plates (6, 12, 24, 96 or 384 wells) or slides.
    Sterile sorts with possibility to expand the sorted cells in culture
    Intracellular or cell-surface staining, fixed or alive cells.

    L2 laboratory completely equiped : hood (subject to booking), centrifuge, pipets
    Ideal operating temperatures between 19 and 26°C

    Becton Dickinson FACS Melody

    
    Tarif établissement partenaire: 25.68 €/heure
    Tarif établissement public et privé : 50.75 €/heure
    Brève description :
    Analyzer and cell sorter by flow cytometry - Becton Dickinson with 3 lasers, 2 physical parameters and 8 fluorescences.
    The system does not require the presence of a dedicated engineer, after training each user can use it alone.
    Excitation and detection :

    Excitation laser 405 nm:
    Filter 1 : 428/45 (DAPI, Hoechst)
    Filter 2 : 528/45 (CFP) or 755LP (BV)

    Excitation laser 488 nm :
    Filter 3 : 527/32, 507 LP (FITC, GFP, AlexaFluor 488...)
    Filter 4 : 700/54, 665 LP (PerCP, PerCP-Cy5.5, 7AAD...)
    Cell sorting on two ways for 1.5, 2 or 5 mL tubes.
    Cloning for plates 6, 24, 48, 96, 96 PCR or 384 wells or slides.

    Temperature control for input sample.
    Excitation laser 561 nm :
    Filter 5 : 582/15, (PE, DsRed...)
    Filter 6 : 613/18, 605 LP (mCherry, PE-CF594, PE-Texas Red, PI...)
    Filter 7 : 697/58, 665 LP (PE-Cy5, PE-Cy5.5...)
    Filter 8 : 783/56, 752 LP (PE-Cy7...)
    Inquiries may be sent to This email address is being protected from spambots. You need JavaScript enabled to view it.
    

    CANTO II Becton Dickinson

    
    Partner lab price : 21.78 €/hour
    Public and private lab price: 59.44 €/hour
    Brief description:
    Flow cytometry cell analyzer - Becton Dickinson
    3 lasers, 2 physical parameters and 8 fluorescences

    Controlled by the FACSDiVa software (Becton Dickinson)
    Blue laser excitation- 488nm :
    Filter 1 (530/30BP) : FITC, AF488, GFP, CFSE, BB515
    Filter 2 (556LP - 585/42BP) : PE, PI, Pyronin
    Filter 3 (610LP)
    Filter 4 (655LP - 670LP) : PerCP, PerCP-Cy5.5, PE-Cy5, PE-Cy5.5, 7-AAD
    Filter 5 (735LP - 780/60BP) : PE-Cy7
    Acquisition of 10 000 events/second

    Intracellular or cell-surface staining, fixed or alive cells

    L2 laboratory completely equiped : hood, centrifuge, pipets
    Red laser excitation - 633nm :
    Filter 6 (660/20BP) : APC, AF647, Cy5, TOPRO-3
    Filter 7 (685LP)
    Filter 8 (735LP - 780/60BP) : APC-Cy7, APC-H7, APC-AF750, APC-eF780

    Violet laser excitation - 405nm :
    Filter 9 (450/50) : BD Horizon V450, Pacific Blue, AF405, DAPI, BV421
    Filter 10 (502LP - 510/50BP) : BD Horizon V500, Pacific Orange, AmCyan, BV510
    Ideal operating temperatures between 19 and 26°C

    CANTO II Becton Dickinson

    
    Partner lab price : 21.78 €/hour
    Public and private lab price: 59.44 €/hour
    Brief description:
    FACS Canto 2 Becton Dickinson cytometer
    3 Lasers, 2 morphological parameters and 8 fluorescences
    30 tubes carroussel
    Diva Software
    BLUE LASER 488nm

    Filter 1 (530/30) : FITC, EGFP, CFSE, TO-PRO-1, Alexa 488
    Filter 2 (585/42) : PE, SNARF, DyeCycle Orange, Alexa 546, DiOC
    Filter 3 (670LP) : PE-Cy5.5, PerCP-Cy5.5, Tri-Color, 7AAD, Alexa 700
    Filter 4 (780/60) : PE-Cy7, Alexa 750
    RED LASER 638 nm

    Filter 5 (660/20) : APC, Alexa647, Cy5, TO-PRO-3, SYTOX Red
    Filter 6 (780/60) : APC-Cy7, APC-H7, APC Alexa 750, APC eFluor780
    VIOLET LASER 405 nm

    Filter 7 (450/50) : BD horizon V450, Pacific blue, alexa fluor 405, dapi
    Filter 8 (510/50) : AmCyan, SYTOX Blue, BD horizon V500, Pacific orange,