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Gallios Beckman Coulter


Partner lab price : 21.78 €/hour
Public and private lab price: 59.44 €/hour
Brief description:
Gallios Beckman Coulter Flow Cytometer
3 lasers et 10 fluorescences and 2 physical parameters
32 tube Multi Carousel Loader
Acquisition 25 000 evts/sec

Kaluza Acquisition Software
BLUE LASER 488nm, 22mW


Filter  1 (525/40) : FITC, EGFP, CFSE, TO-PRO-1, Alexa 488 

Filter  2 (575/30) : PE, SNARF, DyeCycle Orange, Alexa 546, DiOC

Filter  3  (620/30) : PE-Texas Red, PI, DiOC

Filter  4  (695/30) : PE-Cy5.5, PerCP-Cy5.5, Tri-Color, 7AAD, Alexa 700
Filter  5  (755 LP) : PE-Cy7, Alexa 750 
RED LASER 638 nm 25mW


Filter 6 (660/20) : APC, Alexa647, Cy5, TO-PRO-3, SYTOX Red

Filter 7 (725/20) : Alexa 700

Filter  8 (755LP) : APC-Cy7, APC-Alexa 750, APC-H7

VIOLET LASER 405 nm
 40mW

Filter 9 (450/40) : Pacific Blue, Alexa 405, PO-PRO-1, DyeCycle Violet
Filter 10 (550/40) : AmCyan, SYTOX Blue

Posted in 12 parameters analyzer

ImageStreamX MkII


Partner lab price : 48.99 €/hour
Public and private lab price: 131.03 €/hour
Brief description:
Imaging flow cytometer ImageStreamX MkII by Luminex. The system is controlled by the acquisition software INSPIRE. Analysis with the dedicated software IDEAS (Image Data Exploration Analysis Software)
3 LASERs : violet (405nm – 120mw), blue (488nm – 200mW), red (642nm – 150mW)
6 detection channels (Brightfield included) : 435-505 nm / 505-560 nm / 560-595 nm /595-642 nm / 642-745 nm / 745-785 nm
3 magnifications : 20x, 40x, 60x
Applications : Intracellular or cell-surface staining, fixed or alive cells to study cell signaling, cell cycle, internalization and co-localization, cell-cell interaction, shape change, immunological synapse, microbiology, parasitology…
Ideal operating temperatures between 20 and 24°C
L2 laboratory equiped with hood, fridge, centrifuge, pipets,

Posted in Imaging flow cytometry

ImageXpress Pico


Partner lab price : 17.47 €/hour
Public and private lab price: 82.24 €/hour

Brief description:
Automated epifluorescent widefield microscope. Ideal for medium throughput image acquisition and analysis with a large number of samples (cellules, sphéroïdes...) fixed or live and in microplate format. Color and time-lapse imaging possible.
Piloted by CellReporterXpress
Objectives Leica:
4X/NA 0.13 FLUOTAR
10X/NA 0.32 FLUOTAR
20x/NA 0.40 FLUOTAR
40x/NA 0.60 FLUOTAR
Excitation Source:
6 LEDs : Cy5, TRITC, FITC, DAPI, Texas Red, CFP, white light (and RGB)

Filter cubes:
DAPI
FITC
TRITC
Cy5

Can read any format of microplate (6 à 384 wells) and conventional microscopy slides (x4)
Detector:
CMOS camera 5Mpx 12bits
(2000 x 2000 pixels image)

Environmental control: temperature, humidity and CO²
Applications:
-multidimensional automated acquisition: YXZ, multi-channels, multi-fileds, multi-wells, multi-plates, timelaps
-easy image analysis (on-the-fly): 25 preconfigured protocols: Cell counting, Multi-channel Cell scoring, Translocation, Neurite tracing...
-On-the-fly stitching and deconvolution (2D)
-hardware and image autofocus

Posted in High Content Screening

Inverted 1 Zeiss Axio-Observer


Partner lab price : 5.36 €/hour
Public and private lab price: 22.32 €/hour
Associated technologies:
  • 2D imaging
  • Videomicroscopy

    • Brief description:
    Zeiss motorized widefield videomicroscope with incubation chamber for videomicroscpies in 2D with low resolution, reduced frequency, long timelapses in multi-positions, in fluorescence and transmission lights (Bright Field, Phase contrast)
    Controlled by Metamorph software
    Objectives:
    5x Plan Neofluar 0.15 NA PH1
    10X EC-Plan Neofluar 0.3 NA PH1
    20X LD Plan Neofluar 0.4 NA PH2 Korr
    32X LD A Plan 0.4NA PH1 + PLASDIC
    40x LD Plan NEOFLUAR 0.6 NA PH2 Korr

    Detector:
    camera sCMOS Zyla 5.5 (Andor)
    (2560*2160 pixels, 6.4µm pixel size)

    Incubation:
    control of temperature and CO2
    Types of acquisition:
    -epifluorescent widefield acquisition
    -transmitted light acquisition in bright field mode or phase contrast

    The Multi-dimensional Acquisition module in Metamorph controls the multi-series of images in multi-channels, multi-positions, multi-Z planes, multi-timepoints.

    Filter cubes:
    GFP, YFP, Cy3, Cy5
    Illumination sources:
    1 system Lumencor with 4 LEDs:
    GFP, YFP, Cy3, Cy5
    Halogen lamp 100W/12V for transmission
    Compatible sample carriers::
    multi-well plate in plastic, 10, 35, or 60mm diameter dish in plastic

    Continue Reading

    Posted in Videomicroscopy

    Inverted 3 Zeiss Axio Observer

    
    Partner lab price : 5.36 €/hour
    Public and private lab price: 22.32 €/hour
    Associated technologies:
  • 2D imaging
  • Videomicroscopy

    • Brief description:
    Inverted wide-field epifluorescence microscope ideal for 2D or 3D acquisitions followed by deconvolution, on live samples.

    Fluorescence, phase and differential interference contrast (DIC) contrasts
    Multi-dimensional acquisition
    Environmental control (CO2, T°)

    Acquisition software: Zen Blue
    Objectives:
    5X Fluar 0.25NA
    10X EC Plan Neofluar 0.3 NA
    10X LD A-Plan 0.25 NA Ph1
    20X LD A-Plan 0.35 NA Ph1
    20X LD Plan Neofluar 0.4 NA Ph2 Korr

    - - -
    Other objectives available on request :
    10X N-Achroplan 0.25 NA Ph1
    20X LD A-Plan 0.3 NA Ph1
    40X LD Plan Neofluar 0.6 NA Ph2
    Camera: Hamamatsu Orca flash 4.0 LT - B&W, 16-bit, 2048x2048 pixels, pixel size @6.5 µm

    - - -
    Supports:
    Slides
    35 mm Petri dishes
    Culture dishes
    - - -
    Excitation sources:
    Fluorescence: Colibri LED 375 nm, 470 nm, 555 nm
    Transmitted light: LED

    - - -
    Filter cubes:
    DAPI
    GFP
    Rhodamine
    - - -
    Types of acquisition:
    Automated: XYZ, multichannel, mosaic
    Time lapses with environmental control
    Customizable protocol

    Posted in Vidéomicroscopy

    Inverted Nikon

    
    Partner lab price : 15.66 €/hour
    Public and private lab price: 55.41 €/hour
    Associated technologies:
  • 2D imaging
  • Videomicroscopy

    • Brief description:
    Inverted Nikon TiE wide-field microscope for timelapse in 2D or 3D in fluorescence or transmitted light mode (BF, DIC). Trigger option for fast imaging.

    Controled by NIS Elements.
    Objectives:
    10X Plan Apochromat 0.45NA air
    20X Plan Apochromat 0.75NA air
    40X Plan Fluor 1.3NA oil
    60X Plan Apochromat 1.4NA oil
    100X Plan Apochromat 1.40NA oil

    Detectors:
    1 camera sCMOS back-illuminated Prime95B Photometrics
    (1200*1200, 11µm pixel size)
    Incubation:
    temperature and CO2 control

    Type of acquisition :
    -widefield epifluorescent microscopy
    -transmitted light microscope in bright field or DIC mode.

    PFS module for control of the z drift during timelapse.

    Trigger option for fast multicolor imaging.
    Illumination source:
    Lumencor with 7 LED and excitation filters for:
    DAPI, CFP, GFP, YFP, Cy3, Cherry, Cy5

    Emission filters:
    DAPI, CFP, GFP, YFP, Cy3, Cherry, Cy5
    Compatible sample holders:
    35mm dish with #1.5 glass bottom, glass slide with #1.5 glass coverslip or glass-bottom multi-well plate

    Posted in Videomicroscopy

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